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Assay, Hemolytic Titration

A screening assay for circulating Complement Proteins. Diluted Serum samples are added to antibody-coated Erythrocytes and the percentage of Cell lysis is measured. The values are expressed by the so called CH50, in HEMOLYTIC Complement units per milliliter, which is the dilution of Serum required to lyse 50 percent of the Erythrocytes in the assay.