Assay, Two-Hybrid

Screening techniques first developed in Yeast to identify Genes encoding interacting Proteins. Variations are used to evaluate interplay between Proteins and other molecules. Two-hybrid techniques refer to analysis for protein-protein interactions, one-hybrid for DNA-protein interactions, three-hybrid interactions for RNA-protein interactions or ligand-based interactions. Reverse n-hybrid techniques refer to analysis for Mutations or other small molecules that dissociate known interactions.