Assays, Indirect Immunofluorescence

A form of Fluorescent Antibody Technique commonly used to detect Serum Antibodies and Immune Complexes in Tissues and microorganisms in specimens from Patients with infectious Diseases. The technique involves formation of an Antigen-Antibody Complex which is labeled with Fluorescein-conjugated anti-Immunoglobulin antibody. (From Bennington, Saunders Dictionary & Encyclopedia of Laboratory Medicine and Technology, 1984)