Oligonucleotide Probes
Synonyms
Oligodeoxyribonucleotide Probes
Oligoribonucleotide Probes
Probes, Oligodeoxyribonucleotide
Probes, Oligonucleotide
Probes, Oligoribonucleotide
Synthetic or natural oligonucleotides used in hybridization studies in order to identify and study specific nucleic acid fragments, e.g., DNA segments near or within a specific gene locus or gene. The probe hybridizes with a specific mRNA, if present. Conventional techniques used for testing for the hybridization product include dot blot assays, Southern blot assays, and DNA:RNA hybrid-specific antibody tests. Conventional labels for the probe include the radioisotope labels 32P and 125I and the chemical label biotin.