Enzyme-Linked Immunosorbent Assay

Synonyms

Assay, Enzyme-Linked Immunosorbent

Assays, Enzyme-Linked Immunosorbent

ELISA

Enzyme Linked Immunosorbent Assay

Enzyme-Linked Immunosorbent Assays

Immunosorbent Assay, Enzyme-Linked

Immunosorbent Assays, Enzyme-Linked

An immunoassay utilizing an antibody labeled with an enzyme marker such as horseradish peroxidase. While either the enzyme or the antibody is bound to an immunosorbent substrate, they both retain their biologic activity; the change in enzyme activity as a result of the enzyme-antibody-antigen reaction is proportional to the concentration of the antigen and can be measured spectrophotometrically or with the naked eye. Many variations of the method have been developed.